b'SpecificationsMode ofSemi-drytransferGelDenaturing or native polyacrylamide gels, compatibility either homemade or precastRunningHorizontaldimensionCapacity Up to 2 mini or 1 midi with standard system; up to 4 mini and 2 midi with XL systemGel size Mini (8 x 8 cm), midi (8 x 13 cm)Watch how to use the Power Blotter at thermofisher.com/powerblotterThe Power Blotter System offers high transfer efficiency for a broad range of protein sizesApparatus andPower Blotter System with Power BlotterPower Blotter System with Power Blotter consumables Select Transfer Stacks Pre-cut Membranes and Filters, and Power Blotter 1-Step Transfer BufferTransfer time 710 min 710 minTransfer efficiencyFigure 7. Power Blotter Select Transfer Stacks or Power Blotter Pre-cut Membranes and Filters efficiently transfer high, medium, and low molecular weight proteins. Western blot analysis of several targets (KLH, EGFR, Hsp90, PDI, and cyclophilin B protein) was performed by loading serially diluted HeLa cell lysate with KLH spike (starting at 7.5 g HeLa cell lysate, 7.5 g KLH spike per well, serially diluted 2:3) onto Bolt 412% Bis-Tris Plus gels. Proteins were transferred in 7 minutes using a Power Blotter Select Transfer Stack (left, Cat. No. PB5310; PB3310) or Power Blotter Pre-cut Membranes and Filters stack (right, Cat. No. PB9320; PB7320), and probed with target-specific primary antibodies and fluorescently conjugated secondary antibodies. Images were captured using automatic exposure on an Invitrogen iBright Imaging System.Learn more at thermofisher.com/powerblotter12'