b'Table 2. Comparison of elapsed time for protein transfer with the iBlot 2 Gel Transfer Device to other blotting methods. iBlot 2 Gel Transfer Device Conventional semi-dry transfer Wet transferBuffer preparation 0 min 30 min 30 minSoaking gel in transfer buffer 0 min 20 min 0 minAssembling layers 2 min 10 min 10 minTransfer 7 min 4590 min 13 hrCleanup 0 min 10 min 10 minTotal elapsed time 9 min 1 hr, 55 min2 hr, 40 min 1 hr, 50 min3 hr, 50 minTime saved with the iBlot 2NA 1 hr, 45 min 2 hr, 30 min1 hr, 40 min 3 hr, 40 min Gel Transfer DeviceSpecificationsMode ofDrytransferGelNuPAGE Bis-Tris and Tris-Acetate, Bolt compatibility Bis-Tris Plus, Novex Tris-Glycine, and E-PAGE gelsRunningHorizontaldimensionCapacity Up to 2 mini gels or 1 midi gelGel size Mini (8 x 8 cm), Midi (8 x 13 cm)Recommended products Did you knowWe recommend the use of iBlot 2 Transfer Stacks withAlthough nitrocellulose membranes were originally the iBlot 2 Gel Transfer Device. These are available inused to filter out particles, such as bacteria, they nitrocellulose or PVDF, and in regular or mini-gel size. are now used primarily to bind macromolecules in western and Southern blotting. Macromolecules such as proteins are thought to bind to nitrocellulose membranes by hydrophobic interactions. 15'