b'A 1 2 3 4 5 6 7 8 9 10 B 1 2 3 4 5 6 7 8 9 10Pierce Reversible Protein Stain Ponceau S stainFigure 12. Comparison of Pierce Reversible Protein Stain with Ponceau S stain on PVDF membrane. Unstained protein MW markers were serially diluted (lanes 19), applied to two 420% Tris-glycine SDS-polyacrylamide gels, and electroblotted to PVDF membranes. (A) Blot was stained with Pierce Reversible Protein Stain for 1 minute and destained according to the protocol. (B) Blot was stained with 0.1% Ponceau S stain in 5% acetic acid for 5 minutes and destained according to the published protocol. Lane 10: MW marker.Table 11. Comparison of Pierce Reversible Protein Stain with Ponceau S stain.Pierce Reversible Protein Stain Ponceau S stain Tightly binding, higher-sensitivity general protein stainWeakly binding, low-sensitivity general protein stain Detection limit: 2550 ngDetection limit: 250 ng Turquoise blue bands are photographed easily Red bands are difficult to photograph Turquoise bands do not fade over time, but they can be reversedStained protein bands fade within hours Typical staining time: 60 secondsTypical staining time: 5 minutes Background eliminated quickly with low-pH washNo background elimination stepDid you knowTowbin buffer as described by Harry Towbin in 1979 is still the most widely used wet transfer buffer for western blotting. 27'