b'ContentsOverview of western blotting4Transfer5 Introduction to transfer methods for western blotting5Transfer systems for western blotting6Wet transfer (tank transfer)7Semi-dry transfer10 Dry transfer13Pre-transfer considerations16Choice of transfer system16Building the western transfer sandwich16Nitrocellulose membranes17PVDF membranes18Blotting paper20Protein ladder considerations21 Transfer buffers23Post-transfer25Monitoring transfer efficiency 25'