b'Invitrogen No-Stain Protein Labeling Reagent offers the benefits of TPN and also allows you to use your gel of choice. Labeling the blotted proteins on a membrane after protein transfer is easy (Figure 46). Prepare a working solution of No-Stain Protein Labeling Reagent by adding No-Stain Activator and No-Stain Derivatizer to 1X No-Stain Labeling Buffer, and incubate with the membrane for 10 minutes.1 2 3 4After Process westerntransfer blot orstep image nowPrepare Add No-Stain Add No-Stain Incubate with 1X No-Stain Activator Derivatizer membrane for Labeling Buer 10 minFigure 46. Labeling a protein blot with No-Stain Protein Labeling Reagent after transfer.Since it forms covalent bonds with a portion of the lysinesView how-to video:in the total-protein population, the No-Stain reagent signal is stable, and it can be imaged at the same time as the fully processed immunoblot. It can be used with PVDF or nitrocellulose membranes and is compatible with downstream antibody detection using chemiluminescence or fluorescence methods.68'