b'Fluorescent western blot detection compared to both chemiluminescent and chromogenic detection systems. Historically, the instrumentation Fluorescence-based western blot detection differs fromavailable for fluorescent detection has not been able to other detection systems in that the signal produced is notoffer the sensitivity required by many researchers or was a product of an enzyme reaction, but rather a transientprohibitively expensive. However, with the advancements light emission resulting from the excitation and subsequentin imaging technology, new fluorescent probe release of photons as the excited fluorophore returns backdevelopment, and the reduced cost of both, fluorescent to its normal state (Figure 38). Fluorescence-based westerndetection systems are quickly replacing chromogenic detection can be more quantitative than enyzme-basedand chemiluminescent detection methods in many chemiluminesence detection because the signal is typicallylaboratories. While the detection limits are still not as low more stable over time.as chemiluminescent detection, fluorescent detection has the unique advantage of allowing multiple targets to be Fluorescence-based western blotting is growing inassayed for on the same blot at the same time without the popularity because it offers increased time savings overneed to strip and reprobe (known as multiplexing).chemiluminescent detection and reduced chemical waste Figure 38. Overview of fluorescent western blot detection.Watch a how-to video here.60'