b'Overview of western blottingWestern blotting was introduced by Towbin et al. [1] in 1979 and is now a routine and fundamental technique for protein analysis. Western blotting, also called protein blotting or immunoblotting, uses antibodies to identify specific protein targets after they have been separated by electrophoresis and transferred to a membrane. The specificity of the antibodyantigen interaction enables a target protein to be detected in a complex protein mixture, such as cell or tissue lysate. Western blotting can be used to generate qualitative and semi-quantitative data for a protein of interest. In this handbook, you will find information to help you choose the right protein transfer and detection methods for your western blots. You will also find troubleshooting tips, how-to videos, selection guides, and buffer recipes, to help you achieve optimal results.Separate Transfer DetectFor a complete listing of all available products and more, visit thermofisher.com/western Watch an overview video of western blotting here.4'