b'Bolt Transfer Buffer (20X)Novex Tris-Glycine Transfer Buffer (25X)Invitrogen Bolt Transfer Buffer (20X) is optimized for theInvitrogen Novex Tris-Glycine Transfer Buffer (25X) transfer of proteins from Invitrogen Bolt Bis-Tris Plusis optimized for western blot transfer applications gels to membranes for western blotting. When combinedusing Tris-glycine gels. The buffers are made with with 10% methanol, Bolt Transfer Buffer can be used withhigh-purity reagents and are strictly quality controlled. the Invitrogen Mini Blot Module or XCell II Blot ModuleThe concentrated buffer requires a simple dilution with for wet transfer. It can also be used with Invitrogen Boltdeionized water before use.Antioxidant to enhance transfer of reduced proteins to membranes. Pierce 10X Western Blot Transfer Buffer, Methanol-freeThermo Scientific Pierce 10X Western Blot Transfer Buffer, NuPAGE Transfer Buffer (20X)Methanol-free does not require cooling or the addition of Invitrogen NuPAGE Transfer Buffer (20X) is the buffermethanol. Simply dilute the 10X solution with water and use choice for transfer of proteins from Invitrogen NuPAGEdirectly in tank or conventional semi-dry transfer.Bis-Tris and Tris-Acetate gels to membranes for western blotting. NuPAGE Transfer Buffer can be used with the MiniPower Blotter 1-Step Transfer Buffer Blot Module, XCell II Blot Module, or SureLock TandemInvitrogen Power Blotter 1-Step Transfer Buffer is Midi Blot Module for wet transfer. It can also be used withdesigned for rapid semi-dry transfer of proteins from Invitrogen NuPAGE Antioxidant to enhance transfer ofpolyacrylamide gels (SDS-PAGE) to nitrocellulose or reduced proteins to membranes. PVDF membranes using the Power Blotter System. The highionic strength buffer coupled with a high-current BupH Tris-Glycine Buffer Packs power supply allows for protein transfers in 7 to 10 minutes Each Thermo Scientific BupH Tris-Glycine Buffer Packwhen used with a compatible system, such as the yields 500 mL of 25 mM Tris, 192 mM glycine at a pH ofPower Blotter System. Transfer efficiencies are similar to approximately 8 when dissolved in 400 mL deionized waterconventional western transfer techniques. and 100 mL of methanol.Pierce 10X Tris-Glycine Buffer Thermo Scientific Pierce 10X Tris-Glycine Buffer is a space-saving stock solution that is ideal for quickly preparing standard Tris-glycine (pH 8.5) transfer buffer used for western blotting. Simply dilute with deionized water or 20% methanol. Did you knowHarry Towbins group in Basel, Switzerland, was the first to describe electroblotting of proteins to membranes and the use of secondary antibodies. The method he described in 1979 is the closest technique to what we know as modern-day western blotting. 24'