b'Chemiluminescent AP substratesA variety of AP western blot detection substrates existcations and are not compatible with phosphate buffers that can be used as an alternative to the more popularand divalent cation chelators such as EDTA. For AP-based HRP-based systems. With AP-based detection, reactionwestern blot detection we offer CDP-Star Substrate, rates remain linear, and sensitivity can be improved bywhich delivers picogram-level sensitivity and is compatible allowing the reaction to proceed for longer periods ofwith both traditional X-ray film and western blot imaging time. The activity of AP is not affected by exposure toinstrumentation (Table 20). Choose from a stand-alone antibacterial agents, such as sodium azide or thimerosal,substrate, Invitrogen Novex AP Chemiluminescent so it may be stored for long periods of time in nonsterileSubstrate, or a complete kit such as the Invitrogen environments. Optimal enzymatic activity occurs atWesternBreeze Chemiluminescent Kit that contains all pH 9.09.6; these enzymes are activated by divalentsolutions necessary for your application.Table 20. Properties of Invitrogen AP-based chemiluminescent substrates.CDP-Star SubstrateCSPD Substrate Novex AP Chemiluminescent(0.25 mM Ready-to-Use)CSPD Substrate(0.25 mM Ready-to-Use) Substrate (CDP-Star) with Nitro-Block-II Enhancer(0.25 mM Ready-to-Use) with Nitro-Block Enhancer Detection limit Mid- to low-picogram Mid- to low-picogram High-picogram High-picogramExposure ranges 1 second to 15 minutes 1 second to 15 minutes 2 minutes to 2 hours 2 minutes to 2 hoursRecommended membrane PVDF, nylon Nitrocellulose, PVDF PVDF, nylon Nitrocellulose, PVDFCat. No.WP20002 T2218 T2142 T2217Did you knowTris buffers are recommended when using AP-based western detection because phosphate buffers (e.g., PBS) can function as a substrate for alkaline phosphatase. Watch a how-to video here.Learn more at thermofisher.com/chemisubstrates46'