b'Dry transferDry transfer methods use a transfer sandwich containing innovative components that eliminate use of traditionalDid you knowtransfer buffers. A unique gel matrix (transfer stack) that incorporates buffer is used instead of buffer tanks orThe use of impure methanol instead of analytical grade soaked filter papers (Figure 8). The high ionic density in themethanol can increase transfer buffer conductivity and gel matrix enables rapid protein transfer. During dry blottingresult in poor protein transfer.the copper anode does not generate oxygen as a result of water electrolysis, unlike in wet and semi-dry techniques. This absence of oxygen generation reduces blot distortion. Typically, transfer time is reduced by the shortened distance between electrodes, high field strength, and high current. As dry blotting does not require the setup time of wet or semi-dry transfer, not only is the speed of transfer a major benefit, but the overall time investment is improved.Copper cathode2H 2 O + 2eH 2+ 2OH GelNitrocellulose or PVDF membraneCopper anodeCu Cu 2 2e Figure 8. Schematic of Invitrogen iBlot 2 Transfer Stack for dry western blot transfer. 13'