b'No single blocking agent is ideal for every occasion because each antibodytarget pair has unique characteristics. For this reason, we offer a variety of buffers to suit your western blot conditions. See Table 13 for guidance in selecting the right blocking buffer for your experiment.Table 13. Blocking buffer selection guide.Thermo ScientificBlockingAvailable Select whenproduct agent HighlightsWhen to use formats With medium- to high- Performs well with a wide range ofabundance proteins oroptimizing a Serum andstrong antibody affinityPBS StartingBlock biotin-freeantibodies and antibody combinations TBSnew western blotBlocking Buffer single purified Compatible with streptavidin systemsCurrent blocking bufferPBSTsystem proteinhas high background TBST Blocks in less than 15 minStripping and reprobing western blots Blocks excess nonspecific binding sites to help reduceperformingBlocker FLbackground fluorescencefluorescentFluorescent BlockingSingle purified Works with both nitrocellulose and Imaging and storage of dry10X westernBuffer (10X) protein low-fluorescence PVDF membranes fluorescence blots concentratedetection Detergent-free Blocks in 1530 min High-performance replacement foryou have highhomemade milk blocking buffers in background with western blotting applications Use when high backgroundPBShomemade milkBlockerCasein Purified caseinSingle-protein blocking buffer providesis seen with nonfat milk TBSbuffers fewer chances of cross-reaction with assay components than serum or milk solutions 10% solutions of high-quality BSAUse when targetingtargeting Purified bovine Single purified protein providesphosphoproteins 10X phosphoproteins BlockerBSA serum albumin fewer chances of cross-reaction with Best to use when storingconcentrate(BSA) assay components than serum orreused antibodies in blockermilk solutions Helps minimize or eliminate cross-reactivity associated with protein-based blocking buffersPBS you need aPierce Protein-FreeNon-protein Designed for when sample + antibody Use when protein-basedTBSprotein-freeBlocking Buffer blockingcombinations require the elimination ofblockers cause highPBSTblocker compound all possible exogenous animal proteinsbackground TBSTin the assay system to avoid cross-reaction or quenching of the desired probefunctionDid you knowMilk should not be utilized as a blocking buffer when probing for phosphoprotein targets because milk contains casein, a phosphoprotein, which can cause high background. Learn more at thermofisher.com/blockingbuffers34'