b'Mini Blot Module Leak-free, less-buffer wet transfer system The Invitrogen Mini Blot Module is a wet transfer device used exclusively with the Mini Gel Tank and is designed to make your western transfers simple and easy to perform. The tank accommodates one Mini Blot Module per chamber, or two blot modules total with the side-by-side layout. The universal connection and molded gasket make the blot module easy to use, while the inner core of the blot module allows for use of less methanol-based transfer buffer per wet transfer than other commercially available transfer systems. At the recommended conditions andA Bconstant voltage, proteins can typically be transferred to nitrocellulose or PVDF membranes in 30 to 60 minutes (Figure 4).Features: Universal module designallows modules to fit in either chamber of the tank, simplifying the transfer setup Figure 4. Using Bolt Bis-Tris Plus gel chemistry for electrophoresis followed by wet transfer using the Mini Blot Module results in aUnique gasket sealhelps prevent buffer leakage towestern blot with sharp protein signals corresponding to only full-minimize mess during setup of your western transfer length proteins, whereas a western blot of a Bio-Rad TGX gel shows multiple low molecular weight degradation products. Protein1/2-inch buffer chamberrequires only 220 mL perkinases implicated in cancer (IKKB, HCK, EPHB3, MAPK14, FLT1, and DDR2) were analyzed on (A) a Bolt Bis-Tris Plus gel and (B) a Bio-Rad blot of methanol-based transfer buffer, helping saveTGX Tris-glycine gel. Protein samples were prepared for electrophoresis you money on buffer and disposal costs according to each manufacturers protocol. The purified kinases (50 ng each) as GST fusion proteins, along with MagicMark XP Protein StandardStandard 60-minute transfer protocolacceleratesand purified recombinant GST, were loaded in a Bolt 412% gel and a Bio-Rad TGX 420% gel. The samples were separated and transferred your western workflow so you can get results faster to PVDF membranes using the Mini Blot Module for the Bolt gel or on the Bio-Rad transfer system. Blot detection was performed using an anti-GSTRobust electrodes, sturdy steel platesfor highlyantibody and an Invitrogen WesternBreeze Chemiluminescent Kit.efficient and reliable western transfersSpecificationsMode of transfer WetNuPAGE Bis-Tris, Tris-acetate, View how-to video: Gel compatibility Tris-glycine, Tricine, and Bolt Bis-Tris Plus gels; pour-your-own gels cast with the Invitrogen SureCast systemRunning dimension VerticalCapacity 2 blot modules/mini-gel tank; 1 mini gel/blot moduleGel size Mini (8 x 8 cm)Recommended productsThe Invitrogen PowerEase Touch 350W Power Supply and pre-cut nitrocellulose and PVDF membranes are recommended for use with the Mini Blot Module.Learn more at thermofisher.com/minigeltank8'