b'Phospho-AKTA BiBind Western Device ManualCREBC DiBind Western Device ManualFigure 16. Western blots processed on the iBind device show superior sensitivity compared to western blots processed manually. (A and B) Western blots with phosphorylated AKT (left to right: 30 g500 ng cell lysate load) were processed either on the iBind device or using standard manual western processing protocols as specified by the antibody manufacturer (monoclonal phospho-AKT [pT308] (C31E5E) primary antibody; HRP-conjugated anti-rabbit secondary antibody). The blot processed with the iBind device detected phospho-AKT in 500 ng of cell lysate, while the target was detected in 4 g on the manually processed blot. (C and D) Western blots with cell lysate expressing CREB (left to right: 30 g1 g cell lysate load) were processed either on the iBind device or using standard manual western processing protocols as specified by the antibody manufacturer (polyclonal CREB primary antibody; HRP-conjugated anti-rabbit secondary antibody). The blot processed with the iBind device detected CREB in 6 g of cell lysate, whileWatch how to use the iBind devices at 10 g of lysate was needed to detect CREB on the manually processedthermofisher.com/ibindblot. For all blots, proteins were separated using Bolt Bis-Tris Plus mini gels and transferred to PVDF membranes using the iBlot dry blotting system.31'