b'PVDF membranesFeatures:PVDF membranes are ideal for western blotting High qualityPVDF transfer membranes manufactured applications as well as for amino acid analysis andespecially for protein transfer and western blot sequencing of small amounts of proteins (as little asapplications; more resistant to discoloration than other 10 pmol). PVDF membranes are highly hydrophobic andcommercially available PVDF membranesmust be pre-wetted with methanol or ethanol prior to submersion in transfer buffer. PVDF membranes have DurablePVDF is compatible with most organic a high binding affinity for proteins, with binding likelysolvents, acids, and mild bases; doesnt tear or become occurring via dipole and hydrophobic interactions, and offerbrittle like nitrocellulosebetter retention of adsorbed proteins than other supports. Selectionavailable in 0.2 m and 0.45 m pore PVDF is also less brittle than nitrocellulose and can besizes; available in pre-cut sheets, rolls, ready-to-use stripped and reprobed without a loss of sensitivity orformats with membrane included, or in specialized increased background. device-specific formatsWe offer a variety of PVDF membranes to support most of Versatilecompatible with chemiluminescent, your transfer needs. A comparison of the various offeringschromogenic, and fluorescent western blot detectioncan be found in Table 4.While our 0.2 m PVDF membrane performs well for western blotting, amino acid analysis, and protein sequencing applications, our high-quality 0.45 m PVDF membrane is suited for high-sensitivity and low-background immunoblotting. Table 4. Thermo Scientific and Invitrogen PVDF membranes.Pore sizeMembrane0.2 m 0.45 msizeMembrane with filter papers Membrane only Membrane with filter papers Membrane onlyMini PVDF/Filter Paper Sandwiches,Low-Fluorescence PVDFInvitrolon PVDF/Filter Paper Sandwiches,PVDF Transfer Membranes, 0.2 m, 8.3 x 7.3 cmTransfer Membranes, 0.2 m,0.45 m, 8.3 x 7.3 cm 0.45 m, 10 x 10 cm (Cat. No. LC2002) 7 x 8.4 cm (Cat. No. 22860) (Cat. No. LC2005) (Cat. No. 88585)Midi Invitrolon PVDF/Filter Paper Sandwiches, 0.45 m, 8.5 x 13.5 cm(Cat. No. LC2007) NANANASureLock Tandem Midi Pre-cut Membranes and Filters, 0.45 m, PVDF, 8.6 x 14 cm (Cat. No. STM2006)Roll PVDF Transfer Membrane,PVDF Transfer Membrane,NA 0.2 m, 26.5 cm x 3.75 m NA 0.45 m, 26.5 cm x 3.75 m (Cat. No. 88520) (Cat. No. 88518)Table 5. Transfer stacks for the iBlot Gel Transfer Device.MembraneMini size (8 x 8 cm) Regular size (13 x 8.3 cm)sizeNitrocellulose iBlot 2 Transfer Stacks, nitrocellulose, mini (Cat. No. IB23002) iBlot 2 Transfer Stacks, nitrocellulose, regular size (Cat. No. IB23001)PVDF iBlot 2 Transfer Stacks, PVDF, mini (Cat. No. IB24002) iBlot 2 Transfer Stacks, PVDF, regular size (Cat. No. IB24001)Table 6. Transfer stacks and pre-cut membranes with filters for the Power Blotter System.MembraneMini Regular (2 mini or 1 midi)size Pre-assembled stacks Membrane with filter papers Pre-assembled stacks Membrane with filter papersNitrocellulose Power Blotter Select TransferPower Blotter Pre-cut MembranesPower Blotter Select TransferPower Blotter Pre-cut Membranes Stacks, nitrocellulose, miniand Filters, nitrocellulose, miniStacks, nitrocellulose, and Filters, nitrocellulose,(Cat. No. PB3210) (Cat. No. PB7220) regular size (Cat. No. PB3310) regular size (Cat. No. PB7320)PVDF Power Blotter SelectPower Blotter Pre-cut MembranesPower Blotter Select TransferPower Blotter Pre-cut Membranes Transfer Stacks, PVDF, miniand Filters, PVDF, miniStacks, PVDF, regular sizeand Filters, PVDF, regular size (Cat. No. PB5210) (Cat. No. PB9220) (Cat. No. PB5310) (Cat. No. PB9320)18'