b'Pierce CN/DAB Substrate Kit 1-Step Chloronaphthol SolutionThermo Scientific Pierce CN/DAB Substrate KitThermo Scientific 1-Step Chloronaphthol Solution is a includes substrate and peroxide solutions for combinedsolution of 4-chloro-1-naphthol (4-CN) for the chromogenic chloronaphthol- and diaminobenzidine-based detection ofdetection of HRP activity in western blot and tissue staining HRP activity in western blot and tissue staining methods.methods. Chloronaphthol reacts with HRP to form a This kit combines two popular chromogenic peroxidasedistinct blue to blue-purple product to localize and visualize substrate compounds (4-CN and DAB) in a single, stable,the activity of peroxidase-conjugated antibodies on blots 10X stock solution. This CN/DAB solution produces anor fixed-tissue samples. The HRP substrate is not as intense dark black precipitate at sites of bound HRP- sensitive or stable as other chromogenic substrates, such conjugated antibodies on probed blots and tissueas TMB and DAB. However, the blue-purple precipitate samples. The kit includes the 10X solution of CN/DAB andphotographs well, and its alcohol solubility is a property accompanying 1X stable peroxide substrate buffer.that can be utilized in certain double-staining applications.Features: Features:HRP substratefor detection of HRP activity on solid Ready to useno ethanol or methanol required to media via the combined action of 4-CN and DAB dissolve; no dilution necessary for use Chromogenicno special equipment needed for Completedoes not require buffer or addition of visualization; yields intense black bands that are easyhydrogen peroxideto photograph Pre-filtereddoes not need to be filtered before use likeEasy-to-use kitsubstrate stock solution and stablepowders or tablets after they are dissolvedperoxide substrate buffer mean that theres no dry powders to measure and dissolveStablestore refrigerated at 4C for at least one year Stablestore refrigerated at 4C for at least one year58'