b'Detect 28Introduction to western blot detection considerations28Signal-to-noise ratio28Direct vs. indirect detection28Manual vs. automated western blot processing29Automated processing systems30Blocking the membrane33Probing the membrane35Storage conditions for antibodies and antibodyenzyme conjugates39Washing the membrane41Overview of detection methods42 Chemiluminescent western blot detection43Chromogenic western blot detection54Fluorescent western blot detection 60Chemiluminescence and fluorescence detection data capture62Normalization and quantitation in western blot detection67Post-detection and signal enhancement70Stripping and reprobing blots70Signal enhancements75Specialty detection reagents77Appendix79Protocol and recipe quick references79Troubleshooting tips80General western blotting80Fluorescent western blotting82Ordering information84Handbook puzzle challenge90'