b'Signal enhancementsThere are many ways to increase the sensitivity of a western blot. Some methods are as simple as switching detection substrates or blocking buffers in the immunodetection protocols, while others are more time-consuming, such as optimizing antibody titer.A number of post-transfer membrane treatments also exist that can improve signal intensity. Some of these methods increase sensitivity, decrease background, or both; others conserve the amount of antibody needed downstream while maintaining signal intensity.SuperSignal Western Blot Enhancer Increase signal-to-noise ratio and band development for better sensitivityThermo Scientific SuperSignal Western Blot Enhancer contains a membrane treatment reagent and a primary antibody diluent that increases both signal intensity and sensitivity 3- to 10-fold compared to a detection performedFeatures:without it.Increase sensitivityachieve 3- to 10-fold increase in signal intensity and sensitivityWhen a protein is difficult to detect because of low abundance or poor immunoreactivity, use of SuperSignal Improve specificityhelps improve signal-to-noise ratio Western Blot Enhancer can help significantly reducefor poor-quality and low-affinity antibodiesbackground and enhance detection. Reduce backgroundhelps improve clarity for cleanerwestern blots It minimizes the routine problem of overexposing blots, thereby reducing the need to experiment with multiple Membrane compatibilityprovides effective signal exposure times to acquire the perfect blot. This kitenhancement with PVDF and nitrocellulose membranes works with both PVDF and nitrocellulose membranes Substrate compatibilityverified for use with and is compatible with fluorescence, chromogenic, andchromogenic, chemiluminescent, and fluorescent chemiluminescent detection (Figures 5355). detection methodsLearn more at thermofisher.com/supersignalenhancer75'