b'kDa kDakDa kDa kDa 250 kDa200 500 ~180100 150120 130 ~130Gel: 816% Tris-glycine (SDS-PAGE) Blot38% Tris-Acetate GelNuPAGE (SDS-PAGE) Visible light Blot: Chemiluminescent detection Blot: Near-IR detection Gel: 412% Tris-glycine (SDS-PAGE) Blot100 29085 240 95Gel: 18% Tris-tricine (SDS-PAGE) 30 70 80 ~10025 60 7020 50 160 ~7015 55 ~5540 116 43~4010 30 97 34 ~353025 66 26 ~255 2055 ~153.4 15 1510 40 10 ~10Gel: 420% Tris-glycine (SDS-PAGE)Thermo ScientificThermo ScientificInvitrogen HiMarkInvitrogen iBright Thermo Scientific PageRuler PageRulerUnstainedPrestained PageRuler Unstained UnstainedProtein Standard Protein Ladder Prestained Low RangeProtein Ladder Cat. No. LC5688 Cat. No. LC5615 Protein LadderProtein Ladder Cat. No. 26614 Cat. No. 26616Cat. No. 26632kDa kDa kDa kDa kDa kDa 412% Bis-Tris NuPAGE Blot: Invitrogen Gel (SDS-PAGE) chemiluminescent detection460 ~260 38%700 nm and 550 nm uorescent detection ~250 268 ~300 220Gel: 420% Tris-glycine (SDS-PAGE) Blot InvitrogenTris-Acetate Gel (SDS-PAGE) Gel: 420% Tris-glycine (SDS-PAGE) Blot Gel: 412% Tris-glycine (SDS-PAGE) Blot238 ~140 ~250 120~130 ~100 ~180 100~100 171 ~40 80~70 ~25 ~70 ~130 60~55 117 ~15 ~50 50~100 NuPAGE ~10 ~40 40~35 71 ~5 ~35 ~70~25 ~2 ~25 30Visible light55 ~50~15 ~15 ~40 2041~10 31 Invitrogen Novex 16% Tricine Gel ~10Thermo Scientific Invitrogen HiMarkThermo Scientific Thermo Scientific Thermo Scientific Invitrogen MagicMarkPageRuler Plus Pre-stained Spectra Multicolor Spectra Multicolor Spectra Multicolor XP WesternPrestainedProtein Standard Low RangeBroad RangeHigh RangeProtein StandardProtein Ladder Cat. No. LC5699 Protein Ladder Protein Ladder Protein Ladder Cat. No. LC5602Cat. No. 26619 Cat. No. 26628 Cat. No. 26634 Cat. No. 26625pH 310 IEF Protein Gel Novex Gel: InvitrogenkDa kDa pl250 10.7412% Bis-Tris Gel155 9.5130 98 8.395 63 8.07.870 7.455 406.93243 NuPAGE with MES SDS buffer34 21 6.0Visible light Near-IR imager Invitrogen26 5.35.215 11 4.5 Techniques and tools for publication-quality results11 4.23.5Gel: 420% Tris-glycine The(rSmDoS -SPcAieGnEt)ificInvitrogen BenchMarkInvitrogen IEFPageRulerFluorescent Marker 310Prestained NIRProtein Standard Cat. No. 39212-01 For additional information on protein Protein Ladder Cat. No. LC5928Cat. No. 26635420% Tris-glycine ladders, refer to the protein gel (SDS-PAGE) electrophoresis technical handbook at thermofisher.com/pagehandbook or thermofisher.com/proteinladders22'