b'Guardian Peroxidase Conjugate Stabilizer/Diluent Thermo Scientific Guardian Peroxidase Conjugate Stabilizer/Diluent preserves the functional integrity and activity of HRP-conjugated antibodies and other proteins100Oursat very dilute concentrations for long-term storage.90 TheirsWith Guardian Peroxidase Conjugate Stabilizer/Diluent,80typical 1 mg/mL antibody or streptavidin peroxidase70HRP activity (%)conjugates can be diluted as much as 100,000-fold for60storage at 4C. The Guardian solution enables these 50working concentrations of HRP conjugate to be prepared in advance and stored at 4C for 12 months or at room40temperature for 6 months. For blotting applications, it is30usually best to prepare a concentration for storage that20can be diluted 10-fold in assay buffer (which may contain10blocking components) for final use. 01 3 8 15Storage time at 50C (days)Features:Figure 20. Better stability of HRP conjugate with GuardianPreserves HRP activityno significant loss of HRPPeroxidase Conjugate Stabilizer/Diluent. Streptavidin-HRP (1 mg/mL) activity over a 6 month period at room temperaturewas diluted 1:1,000 and stored at 50C in Guardian stabilizer/diluent (Ours) or another suppliers HRP stabilizer (Theirs). At each time point, (1:1,000 dilution) or 12 months at 4C the HRP conjugate was diluted 1:5,000,000 with Thermo Scientific Convenientstore ready-to-use dilutions (1:1,000SuperBlock (PBS) Blocking Buffer and incubated for 1 hour in a white plate that had been coated with biotinylated BSA. The plate was then to 1:100,000) that maintain enzyme activity in thewashed 3 times with 200 L PBST. Thermo Scientific SuperSignal refrigerator; no aliquoting or freezing needed ELISA Femto Substrate (100 L) was added to the plate and incubated for 1 minute and then measured in a luminometer. Samples at each timeAssay compatiblesimply add your favorite blockingpoint were compared to control (i.e., HRP conjugate that had not been stored in diluted form). Storing enzymes at 50C for 2 weeks is equivalent buffer to create the ideal diluent for your HRP-basedto 12 months at 4C.ELISA system or store the HRP conjugate as a 1:1,000 stock solution for western blots and dilute in the final assay buffer Helps save moneyless expensive than alternative formulations or ordering new HRP conjugates Superior stabilitybetter maintenance of HRP activity over time (Figure 20)Did you knowDetergents such as Tween 20 surfactant can help minimize background when added to a blocking buffer, but too much detergent can prevent sufficient blocking. A final concentration of 0.05% often works well but may need to be optimized for the specific application.40'