b'Restore PLUS Western Blot Stripping BufferWhen researchers require a robust but gentle western blotting stripping buffer, the original Restore Western Blot Stripping Buffer has been the buffer of choice. However, some antibodies remain difficult to remove from chemiluminescent western blots and require longer incubation times or incubation temperatures greater than 22C. Thermo Scientific Restore PLUS Western Blot Stripping Buffer was developed to reduce incubation times while keeping incubations at room temperature using gentler formulations. High-affinity antibodies can be quickly and effectively stripped from western blots withoutRestore PLUS bu\x0fer Supplier A Supplier Cremoving transferred proteins, allowing multiple reprobes of1 2 3 1 2 3 1 2 3the target.Original ActinFeatures: Ready and easy to useno dilution necessary; no offensive odors; store at room temperatureActinStripped Compatibleuse on nitrocellulose and PVDF membranes, whether still wet or already dry; works with practically any blocking buffer, enzyme conjugate, andActinchemiluminescent substrate ReprobedCyclophilin B Cost effectivesave valuable time and samples; strip blots effectively the first time Figure 51. Restore PLUS Western Blot Stripping Buffer is most effective for reprobing with different antibodies. HeLa cell lysate wasRobust yet gentletransferred proteins remainprobed for actin and detected with Pierce ECL substrate (Original). Blots functional; strip the same blot up to 5 times were then stripped with either Restore PLUS Western Blot Stripping Buffer or other suppliers stripping buffers. The blots were then reblocked andFlexiblestrip and reprobe to optimize antibodyreprobed for cyclophilin B and detected with Pierce ECL substrate.concentrations or to detect a new target protein with different antibodies (Figure 51)Watch a how-to video here.73'