b'iBlot 2 Gel Transfer DeviceDry western transfer in 7 minutes, with no transfer buffer neededThe Invitrogen iBlot 2 Gel Transfer Device is for dry electroblotting of proteins from mini-, midi-, and Invitrogen E-PAGE gels onto nitrocellulose or PVDF membranes for western detection. The iBlot 2 system offers exceptional transfer efficiency, convenience, and speed, producing crisp and clear bands that remain sharp and straight (Figure 9). Buffer ion reservoirs incorporated into the gel matrix (transfer stacks as opposed to buffer tanks or soaked filter papers) enable rapid protein transfer toA B Ceither nitrocellulose or PVDF membranes. The shortened distance between electrodes, along with high field strength and current, reduce run times to 7 minutes (Table 2). With the iBlot 2 system, there is no need to prepare buffers, pretreat your gel, presoak filter papers, or clean up after blotting. Bolt Bis-Tris Plus,NuPAGE Bis-Tris,Novex Tris-glycine, NC NC NCD E FFeatures: Engineered for rapid transferthe short distance between electrodes, along with high field strength and current, reduces transfer time to just 7 minutes Minimal preparation and cleanuptransfer stacksBolt Bis-Tris Plus,NuPAGE Bis-Tris,Novex Tris-glycine, streamline transfer setup and teardown PVDF PVDF PVDFFigure 9. Membranes processed on the iBlot 2 Gel Transfer DeviceConvenienttouchscreen interface, preprogrammedshow consistent transfer across various protein gel chemistries to and optimized transfer protocols, and prepackagedboth nitrocellulose (NC) and PVDF membranes. Total cell extracts from A431 cells were transferred to NC (AC) or PVDF (DF) membranes from ready-to-use stacks for transferring midi and mini blotInvitrogen Bolt 412% Bis-Tris gels, Invitrogen NuPAGE 420% Bis-Tris formats (1 midi blot or up to 2 mini blots at a time) gels, and Invitrogen Novex 420% Tris-glycine gels. View how-to video:Learn more and see the video at thermofisher.com/iblot214'