b'Pierce NBT and BCIP substrates WesternBreeze AP chromogenic kits The Invitrogen WesternBreeze chromogenic kits yield Thermo Scientific NBT and BCIP substrates are powdershigh-sensitivity results with extremely low background and ready-made solutions of nitro blue tetrazolium (NBT)without the need for any additional equipmentjust watch and 5-bromo-4-chloro-3-indolyl phosphate (BCIP) forthe signal develop over a short period of time. The kit uses chromogenic blot and IHC staining with an alkalinethe chromogenic phosphatase substrate BCIP and phosphatase probe, yielding an intense, insolubleelectron-transfer agent NBT to produce a dark blue black purple precipitate. The NBT and BCIP powders areprecipitate at the precise site of enzymatic activity on available individually and in two convenient, ready-to-usethe blot.Thermo Scientific 1-Step NBT/BCIP substrate solutions (with and without a levamisole suppressor of endogenousThe WesternBreeze kit has been optimized to provide phosphatase activity). picogram sensitivity. Each kit includes ready-to-use and easy-to-dilute blockers, primary antibody diluent, wash Features:solution, conjugated secondary antibody solution (eitherBest for immunoblotting applications AP-conjugated anti-mouse, anti-rabbit, or anti-goat), substrate, and two convenient incubation trays. Sensitive with low background and minimal assay-to-assay variability Features: Choose individual NBT and BCIP substrate powders, or Clear backgroundpreformulated, ready-to-use 1-Step NBT/BCIP substrate High sensitivity, low picogram levels detectablesolutions, with or without levamisole suppressor High specificity One simple protocol, no optimization required59'