b'Blotting paper Pierce Western Blotting Filter Papers Blotting (or filter) paper is an essential component for theThermo Scientific Pierce Western Blotting Filter Papers transfer sandwich in wet and semi-dry transfer methods.are pre-cut cotton sheets to support wet or semi-dry The filter paper is first wetted in transfer buffer beforeelectrophoretic transfer of proteins from polyacrylamide building the transfer sandwich. The paper serves to aidgels (SDS-PAGE) to PVDF, nitrocellulose, or other the wicking of transfer buffer through the gel, helpingmembranes (Table 8). Pierce Western Blotting Filter the proteins move out of the gel onto the membrane.Papers are suitable for use with alcohol or other organic Dry electrotransfer conditions do not use filter paper. solvents commonly used in protein and nucleic acid blotting applications.Filter paper should be made of high-quality materials so that it doesnt contribute to possible backgroundFeatures:issues during the western blotting detection step. High qualityclean cotton cellulose fiber paper The paper thickness may also be of concern with somemanufactured with additive-free ultrapure water to transfer systems. For example, in a semi-dry transfer setup,minimize sources of background signal and artifactsthe thickness of the filter paper can affect how much buffer reservoir is available for transfer. A thicker filter paper can Easy to usepre-cut filter paper sheets in several typically hold more transfer buffer. When using a transferconvenient sizes for use with most mini gel sizes, tank device, it is important to follow the vendors recommendedtransfer cassettes, and semi-dry blottersfilter paper thickness.Verifiedtested for use with various protein methods, including wet and semi-dry transfer Two thicknessesstandard-thickness filter paper for traditional procedures; extra-thick filter paper for high-capacity blotting or as a replacement for multiple sheetsTable 8. Pre-cut filter papers.Thickness Mini size Midi size Special sizes0.83 mm Western Blotting Filter Paper, Western Blotting Filter Paper, Western Blotting Filter Paper,7 cm x 8.4 cm 8 cm x 13.5 cm 8 cm x 10.5 cm(Cat. No. 84783) (Cat. No. 84784) (Cat. No. 88600)2.5 mm Western Blotting Filter Paper, Western Blotting Filter Paper,Western Blotting Filter Paper, Extra Thick, 7 cm x 8.4 cm Extra Thick, 8 cm x 13.5 cm Extra Thick, 8.5 cm x 9 cm(Cat. No. 88605) (Cat. No. 88615) (Cat. No. 88610)Blotting Filter Papers, 2.5 mm thick,Blotting Filter Papers, 2.5 mm thick,Western Blotting Filter Paper, 7.5 cm x 8.4 cm 8.6 cm x 13.5 cm Extra Thick, 20 cm x 20 cm(Cat. No. LC2010) (Cat. No. LC2008) (Cat. No. 88620)Did you knowThe addition of 0.05% SDS to the transfer buffer helps with transfer of large molecular weight proteins.Learn more at thermofisher.com/filterpaper20'