b'Automated processing systemsiBind western systemsThe iBind western systems are nonpowered devices thatFeatures: automate immunodetection steps. The traditional manual Flexibilitypick the system that matches your process involves preparing and replacing multiple antibodythroughput; process 1 mini or midi blot, 2 mini and wash solutions over several hours in a tray containingblots, or 6 vertically cut strips using the same or the blot of interest. By contrast, iBind western systemsdifferent conditionsallow all solutions to be prepared and loaded in the device at the start of the procedure (Figure 15). Subsequent steps Antibody savingsuse up to 80% less primary antibodyproceed automatically and uninterrupted by sequential Load and gothe system processes solutions using lateral flow (SLF) technology, i.e., simple capillary action SLF technology, with no batteries, shakers, trays, or no electricity or batteries are required. timers requiredTwo iBind western systems are available: the original Reproducibilityautomated blot processing enables Invitrogen iBind Western Device, which accommodatesimproved blot-to-blot consistencyone mini blot at a time, and the Invitrogen iBind Flex SensitivityiBind systems offer greater sensitivity than Western Device, which accommodates up to one midi blot,manual methods for many monoclonal and polyclonal two mini blots, or up to six vertically cut strips at a time.antibodies (Figure 16)Table 12 compares the two systems.Row/well Solution1 Primary antibody2 1X iBind/iBind FD Solution3 Secondary antibody4 1X iBind/iBind FD SolutionFigure 15. Sample loading wells for the iBind western system.Table 12. Characteristics of iBind western systems.iBind Western Device iBind Flex Western DeviceMini blot (8 x 8 cm), single Yes YesMini blot (8 x 8 cm), dual No YesMidi blot (13 x 8 cm) No YesVertically cut strips (up to 6) No Yes30'