b'Bolt Bis-Tris Plus and NuPAGE Bis-Tris gelsNeutral-pH gel systems for optimal separationBolt Bis-Tris Plus and NuPAGE Bis-Tris gels are precastBolt Bis-Tris Plus and NuPAGE Bis-Tris gels offer:polyacrylamide gels designed for optimal separation of a Preserved protein integrityneutral-pH formulation broad molecular weight range of proteins under denaturingminimizes protein modifications (Figure 8)conditions during gel electrophoresis (Figures 610). These gels help deliver consistent performance with a Superior band quality and band volumeBis-Tris gel neutral-pH environment to minimize protein degradation,chemistry is designed to deliver sharp, straight bands resulting in sharper bands without typical gel smiling.with higher band volumeAdditionally, preserving protein integrity becomes High sample volume capacitywedge-well particularly important when separating low-abundancedesign of Bolt Bis-Tris mini gels allows detection of proteins. The unique wedge-well design of Bolt Bis-Trisproteins in very dilute samples or measurement of Plus gels (Figure 5) allows loading up to twice the samplelow-abundance proteinsvolume of other precast gels. Bolt Bis-Tris Plus and NuPAGE Bis-Tris gels are ideal for western blot transfer More efficient western blot transferneutral pH and analysis along with any other technique where proteinprevents reoxidation of reduced samples during integrity is crucial. Bolt Bis-Tris Plus gels are available in theprotein transfermini gel format and NuPAGE Bis-Tris gels are available in High lot-to-lot consistencycoefficient of variation (CV) mini and midi gel formats, as well as multiple thicknesses. of only 2% for Rf values (migration)sults acquired witRe hthe Mini Gel TankFigure 6. Bolt Bis-Tris Plus gel electrophoresis. Protein standards and samples were loaded at 10 L sample volumes in a Bolt 412% Bis-Tris Plus gel. Electrophoresis was performed using the Mini Gel Tank at Figure 5. Unique wedge-shaped well design of Bolt Bis-Tris Plus gels.200 V (constant). Sharp, straight bands with consistent migration patterns The WedgeWell feature enables larger sample loads and easier loading.were observed after staining with Invitrogen SimplyBlue SafeStain. Images were acquired using a flatbed scanner. Lane 1: Invitrogen SeeBlue Plus2 Prestained Standard; lane 2: 10 g E. coli lysate; lane 3: Invitrogen Mark12 Unstained Standard (blend of 12 purified proteins); lane 4: 40 g HeLa cell lysate; lane 5: 20 g HeLa cell lysate; lane 6: 5 g BSA; lane 7: 40 g Jurkat cell lysate; lane 8: 5 g GST fusion protein; lane 9: Invitrogen Novex Sharp Unstained Protein Standard; lane 10: 5 g -galactosidase.View the video to see how easy it is to run an Invitrogen gel here.Review the quick reference protocol for Bolt gels here.Review the quick reference protocol for NuPAGE Bis-Tris mini gels here.Learn more at thermofisher.com/nupage16'