b'1. Snap the electrophoresis tank into the base, and2.Remove the comb, and peel away the tape at the3.Place the cassette in the chamber with the wellsplace the cassette clamp(s) into the chamber(s) bottom of the gel cassette. facing towards you.with the anode connector(s) (+) aligned to the center. Rinse the wells 3 times with 1X bu\x02er. Hold the cassette in a raised position and close Fill the chamber(s) with 1X bu\x02er to the level ofthe clamp by moving the cam handle forward.the cathode.4.Make sure the wells are completely lled with5.Hold the cassette and release the cassette clamp. 6.Make sure the power supply is o\x02.1X bu\x02er.Gently lower the casette so that it rests on theIf only running one gel, remove the cassette Load your samples and markers. bottom of the chamber, and close the cassette clamp. clamp from unused chamber.Add 1X bu\x02er to the level of the ll line. Place the lid on the tank and plug theelectrode cords into the power supply.Turn the power supply on to begin electrophoresis.Figure 25. How to use the Mini Gel Tank. Figure 26. Electrophoresis of Bolt gel using the Mini Gel Tank. Protein standards and samples were loaded at 10 L sample volumes on an Invitrogen Bolt 412% Bis-Tris Plus gel. Electrophoresis was performed using the Mini Gel Tank at 200 V (constant). Sharp, straight bands with consistent migration patterns were observed after staining with Invitrogen SimplyBlue SafeStain. Images were acquired using a flatbedRecommended productsscanner. Lane 1: SeeBlue Plus2 Prestained Standard; lane 2: 10 g E. coli lysate; lane 3: Invitrogen Mark12 Unstained Standard (blend of 12The Invitrogen Mini Blot Module is a wet transfer purified proteins); lane 4: 40 g HeLa cell lysate; lane 5: 20 g HeLa cellapparatus that conveniently fits into the chambers of the lysate; lane 6: 5 g BSA; lane 7: 40 g Jurkat cell lysate; lane 8: 5 g GST fusion protein; lane 9: Invitrogen Novex Sharp Unstained ProteinMini Gel Tank to easily transfer proteins from mini gels to Standard; lane 10: 5 g -galactosidase. nitrocellulose or PVDF membranes. 55'