b'Western blot protein ladders Western blotspecific protein ladders are designed for easyRecommended for: and convenient protein molecular weight estimation directly on western blots or indirectly by using various blotting Approximate molecular weight determination directly on detection systems (e.g., chemiluminescent, fluorescent, orwestern blots or indirectly through chemiluminescent, chromogenic). The protein markers consist of recombinantfluorescent, or chromogenic detection systemsproteins with an IgG binding site. The IgG binding site Qualitatively assessing transfer efficiencybinds the primary or secondary antibody used for detection of the target protein, allowing visualization of the standard on the western blot.Indirect detectionFluorescent detection Indirect and direct detectionkDa kDa kDa kDa250 250220120 130 130100 9580 95 80Visible light Visible light Chemiluminescent detection Near-IR detection60 70 7050 55 5540 43 4334 34Near-IR detection3030 26 2620 15 1511 10NuPAGE Gel: 420% Tris-glycineNovex412% Bis-Tris Gel (SDS-PAGE) 420% Tris-Glycine GelInvitrogen MagicMark XP WesternThermo Scientific PageRuler Prestained NIRInvitrogen iBright PrestainedProtein Standard Protein LadderProtein LadderCat. No. LC5602 Cat. No. 26635 Cat. No. LC5615Visualization: user-defined detectionVisualization: colorimetric, NIR fluorescenceVisualization: colorimetric, NIR fluorescence system (not recommended for RGB(670 nm red laser or 700 nm channel) (700 nm channel), user-defined detection system fluorescence systems) (2 bands)49'