b'Protein gel electrophoresis chamber systemsMini Gel Tank troubleshootingObservationPossible causeSuggested solution Buffers are too diluteCheck buffer recipe; dilute from concentrate or remake if necessary. Run taking longer than usual Buffer chamber is leaking Make sure the cassette clamp is firmly seated, the gaskets are in place, and the cassette clamp is locked.Current is set too low Set correct current.Tape left on the bottom of the cassette Remove tape from bottom of cassette.Current reading on powerConnection to power supply not complete Check all connections with a voltmeter for conductance.supply is zero or very low Make sure there is sufficient buffer in the electrophoresis tank to cover Insufficient buffer level the wells of the gel.Run is faster than normalBuffers are too concentrated or incorrect Check buffer recipe; dilute or remake if necessary.with poor resolution Current is set at a higher limit Decrease current to recommended running conditions (see page 62).Cannot see the sample wellsThere is little contrast between theMark cassette at the bottom of the wells with a marker pen prior to to load sample sample well and the rest of the gel placing the cassette in the electrophoresis tank.XCell SureLock Mini-Cell troubleshootingObservationPossible causeSuggested solution Buffers are too diluteCheck if buffer was diluted properly. Check buffer recipe; dilute from concentrate or remake if necessary. Run taking longer than usual Upper buffer chamber is leaking Make sure the buffer core is firmly seated, the gaskets are in place, and the gel tension lever is locked.Voltage is set too low Set correct voltage.Tape left on the bottom of the cassette Remove tape from bottom of cassette.Current reading on powerConnection to power supply not complete Check all connections with a voltmeter for conductance.supply is zero or very low Make sure the upper buffer (cathode) is covering the wells of the gel. Insufficient buffer level Be sure there is sufficient buffer in the lower buffer chamber to cover the slot at the bottom of the gel.Run is faster than normalBuffers are too concentrated or incorrect Check buffer recipe; dilute or remake if necessary.with poor resolution Voltage, current, or wattage is set at aDecrease power conditions to recommended running conditions higher limit (see page 62).Cannot see the sample wellsThere is little contrast between theMark cassette at the bottom of the wells with a marker pen prior to to load sample sample well and the rest of the gel assembling the upper buffer chamber. Illuminate the bench area with a light source placed directly behind the XCell SureLock unit.77'