b'NuPAGE Bis-Tris gelNuPAGE Bis-Tris gels (denaturing separation)specifications Shelf life: 12 months Average run time: 35 minutes Separation range: 1.5300 kDa Polyacrylamide concentrations: fixed 8%, 10%, and 12%; gradient 412% Gel dimensions: Mini: 8 x 8 cm (1 or 1.5 mm thick) Midi: 8 x 13 cm (1 mm thick) Maximum sample volume per 10-well mini gel: 25 L (1 mm thick); 37 L (1.5 mm thick)Recommended productsThermo Scientific PageRuler, PageRuler Plus, and Spectra prestained protein ladders are recommended for use with NuPAGEBis-Tris gels for easy molecular weight determination.Visualize with Coomassie stain, silver stain, or fluorescent protein stains after electrophoresis (see Staining gels on page 63).Figure 10. Migration patterns in NuPAGE Bis-Tris gels using Invitrogen Novex Did you know Sharp Prestained Protein Standard or Novex Sharp Unstained Protein Standard. Timothy Updyke and SheldonThe optimal separation range is shown within the gray areas.Engelhorn filed a patent for the neutral-pH Bis-Tris gel system in 1996.Review the quick reference guide for NuPAGE Bis-Tris Mini Gels here.19'