b'Novex Zymogram gel10% gel (with gelatin)102030140Percent length of gel50607080 2903100Figure 22. Novex Zymogram Plus (gelatin) gel migration chart. The numbered bands refer to the following proteases:Band 1: Collagenase type I (140 kDa) Band 2: Thermolysin (37 kDa)Band 3: Trypsin (19 kDa)Recommended productsAfter electrophoresis, incubate the gel in Novex Zymogram Renaturing Buffer (10X) to renature the enzyme. The gel is then equilibrated in Novex Zymogram Developing Buffer (10X) to add divalent metal cations required for enzymatic activity.31'