b'ContentsElectrophoresis overview4 Choosing the right protein gel8Bis-Tris chemistry vs. Tris-glycine chemistry8 Gel selection guide11Premade gels16Handcast gel system34 Preparing samples and selecting buffers36Protein extraction and clean-up methods37Protein quantitation40 Electrophoresis buffers and reagents42 Buffer recipes43Estimating protein sizes46Protein ladders and standards46Choosing the electrophoresis chamber and power supply52Electrophoresis chamber system selection guide53Electrophoresis chambers 54Power supplies61 Running gels62Gel running conditions62Staining gels63Protein stains63Protein stain selection guides68, 70, 71 Western blotting72Transfer and detection72Appendix74Protocol quick references74 Troubleshooting tips75 Ordering information79Handbook puzzle challenge88'