b'ults ac MagicMark standardGST IKK EPHB3 HCK MAPK14 FLT1 DDR2 MagicMark standardGST IKK EPHB3 HCK MAPK14 FLT1 DDR2es quired witR hthe Mini Gel TankBolt Bis-Tris Plus gel Bio-Rad TGX gelFigure 7. NuPAGE Bis-Tris gel electrophoresis. Protein standardsFigure 8. Bolt Bis-Tris Plus mini gels help provide better western and samples were loaded at 10 L sample volumes in an Invitrogenblotting results. A western blot of a Bolt gel shows clean, sharp protein NuPAGE 412% Bis-Tris gel. Electrophoresis was performed usingsignals corresponding to only full-length proteins, whereas a western blot the Mini Gel Tank at 200 V (constant). Sharp, straight bands wereof a Bio-Rad TGX gel shows multiple low molecular weight degradation observed after staining with SimplyBlue SafeStain. Images were acquiredproducts. Protein kinases implicated in cancer (IKK, EPHB3, HCK, using a flatbed scanner. Lane 1: SeeBlue Plus2 Prestained Standard;MAPK14, FLT1, and DDR2) were analyzed on a Bolt Bis-Tris Plus gel and lane 2: 10 g E. coli lysate; lane 3: Mark12 Unstained Standard (blenda Bio-Rad TGX Tris-glycine gel. The purified kinases (50 ng each), along of 12 purified proteins); lane 4: 40 g HeLa cell lysate; lane 5: 20 gwith Invitrogen MagicMark XP Western Protein Standard and purified HeLa cell lysate; lane 6: not used; lane 7: 40 g Jurkat cell lysate;recombinant GST protein, were loaded on a 10-well, 412% Bolt gel lane 8: 5 g GST fusion protein; lane 9: Novex Sharp Unstained Proteinand a 10-well, 420% Bio-Rad TGX gel. The samples were separated Standard; lane 10: 5 g -galactosidase. and transferred to 0.45 m PVDF membranes using the respective manufacturers protocols. Immunodetection was performed using an anti-GST antibody and Invitrogen WesternBreeze chemiluminescent NuPAGE Bis-Tris gels have beendetection. The blots were imaged using an LAS-1000 system (FujiFilm).referenced in 20,000 publications.Bolt Welcome Pack17'