b'Novex IEF gelsSeparated on precast vertical gel (slab)310 37 Cathode ()8. 3 6. 98.07.87.46.06.9 6 .0 5.35.2 5.35.2 4.5 Did you know4.2 4.53.5 4.2 Harry Svensson-Rilbe and his student Olof Vesterbergfirst described the theory of separation of amphoteric3.5 proteins along a pH gradient by applying an electric Anode (+) field in the 1960s.Figure 21. Novex IEF gel migration chart using the IEF marker. Proteins shown are amyloglucosidase (Aspergillus niger), pI = 3.5; glucose oxidase (Aspergillus niger), pI = 4.2; trypsin inhibitor (soybean), pI = 4.5; -lactoglobulin (bovine, milk), pI = 5.2 and 5.3; carbonic anhydrase (bovine, erythrocytes), pI = 6.0; myoglobin (horse, muscle), pI = 6.9 and 7.4; lectin (Lens culinaris), pI = 7.8, 8.0, and 8.3; ribonuclease A (bovine, pancreas), pI = 9.5; and cytochrome c (horse, heart), pI = 10.7.Recommended productsInvitrogen Novex IEF buffer kits include optimized cathode, anode, and sample buffers to help reduce variability and enable consistent results.IEF Marker 310 are ready to use and give accurate results.29'