b'SureLock Tandem Midi Gel Tank2-in-1 midi gel electrophoresis and transfer tankThe Invitrogen SureLock Tandem Midi Gel Tank enables rapid electrophoresis of midi gels using minimal buffer (~520 mL/gel) in a leak-free system. With a setup time of ~30 seconds, the tank efficiently runs midi gels while also providing consistent performance. The tank features two independent chambers, allowing electrophoresis of one or two gels at a time, which saves on buffer and limits waste. The SureLock Tandem Midi Gel Tank can be used for wet Double duty instrumentenables electrophoresis and tank transfers when paired with the SureLock Tandemtransfer of high-performance Invitrogen midi gels using Midi Blot Module. The SureLock Tandem Midi Blotthe same tankModule performs efficient, room-temperature wet protein transfers for downstream western blot analysis. The tank Two separate chambersrun 1 or 2 gels or transfers accommodates two blot modules, allowing transfer ofusing only the necessary amount of buffer for each gel, one or two gels at a time, using considerably less transferminimizing buffer cost and wastebuffer (only ~300 mL per transfer) than other wet transfer Room-temperature transfereliminate the need systems. This lower buffer requirement keeps the amountto prechill buffers and the hassle and messiness of of methanol waste (a hazardous material) to a minimum. ice baths User-friendly designeasy setup, sample loading, and simple workflow, without dummy cassettes or buffer dams Compatiblefits all Invitrogen precast midi gels and Invitrogen midi gel cassettesSpecifications Gel capacity: up to 2 midi gels (8 x 13 mm) Cell size (L x W x H): 25 x 17.9 x 17.3 cm(height with lid on)NuPAGE Bis-Tris 412% gradient midi gel Buffer chamber requirement: Figure 29. Publication-quality protein electrophoresis gel results using a NuPAGE Bis-Tris 412% gradient midi gel and the SureLock Upper chamber: 170 mL (per gel)Tandem Midi Gel Tank. The gel was loaded as follows: Lanes 1, 20: 5 L PageRuler Broad Range Unstained Protein Ladder (Cat. No. 26630); Lower chamber: 350 mL (per gel)lanes 27: 10 g, 8 g, 6 g, 4 g, 2 g, 1 g HeLa lysate; lanes 8, 13: 5 L Novex Mark12 Unstained Standard (Cat. No. LC5677); lanes 912: 240 ng, 180 ng, 120 ng, 60 ng of protein mix containing -galactosidase, Chemical resistance: impervious to most alcohols lactate dehydrogenase, and lysozyme; lanes 1419: 10 g, 8 g, 6 g,but not compatible with chlorinated hydrocarbons 4 g, 2 g, 1 g E. coli lysate. Electrophoresis was conducted with(e.g., chloroform), aromatic hydrocarbons (e.g., toluene, NuPAGE MOPS running buffer and stained with SimplyBlue Safe Stain.benzene), acetone, or isopropyl alcoholView performance data and a how-to video at thermofisher.com/surelocktandem58'