b'Bolt Bis-Tris Plus miniBolt Bis-Tris Plus gelsgel specifications Shelf life: 16 months Average run time: 35 minutes Separation range: 0.3260 kDa Polyacrylamide concentrations: fixed 8%, 10%, and 12%; gradient 412% Gel dimensions: 8 x 8 cm (1 mm thick) Maximum sample volume per 10-well gel: ~60 L, or two-thirds of the sample well volume The new Bolt system is wonderful. I am still amazed that I can run a PAGE gel in 23 minutes. The entire system is incredibly user-friendly, from the Bolt precast gels with wedged wells for ease of loading to the Mini Gel Tank system. The bands produced from the westerns were sharp and straight. I would and have highly recommended this system to anyone doing protein work.Crystal M., large Canadian universityFor one of our projects in the lab, we resolve proteins by electrophoresis to determine the accumulation of ubiquitinated proteins following treatment with a proteasome inhibitor. When we resolved the ubiquitinatedFigure 9. Bolt Bis-Tris Plus gel migration chart. Optimal separation range is shown within the proteins using the Tris-glycinegray areas.gels, we observed a smear. However, when we switched to resolving the ubiquitinated proteins using the Bolt Bis-Tris gels, we were delightfully surprised to observe individual protein bands in place of the smear. Susan S., large U.S. university18'