b'NuPAGE Bis-Tris and Tris-Acetate buffer recipesBufferStorageComponentConcentration (1X) NuPAGE LDS Sample Buffer425CGlycerol 10%Tris base 141 mMTris-HCl 106 mMLDS 2%EDTA 0.51 mMSERVA Blue G-250 0.22 mMPhenol red 0.175 mM(pH 8.5)NuPAGE MOPS SDS Running Buffer*425C MOPS 50 mMTris base 50 mMSDS 0.1%EDTA 1 mM(pH 7.7)NuPAGE MES SDS Running Buffer*425C MES 50 mMTris base 50 mMSDS 0.1%EDTA 1 mM(pH 7.3)NuPAGE Tris-Acetate SDS Running Buffer 425C Tris base 50 mMTricine 50 mMSDS 0.1%(pH 8.24)NuPAGE Transfer Buffer425C Bicine 25 mMBis-Tris (free base) 25 mMEDTA 1.0 mMChlorobutanol 0.05 mM(pH 7.2)*The premixed buffers (Cat. Nos. NP0001 and NP0002) also contain trace amounts of the proprietary NuPAGE Antioxidant (Cat. No. NP0005) for stability. Additional antioxidant may be required with specific protocols. Novex Tris-Glycine buffer recipesBufferStorageComponentConcentration (1X) Novex Tris-Glycine SDS Sample Buffer 4C Tris-HCl* 63 mMGlycerol 10%SDS 2%Bromophenol blue 0.0025%Deionized water (pH 6.8)Novex Tris-Glycine Native Sample Buffer 4C Tris-HCl* 100 mMGlycerol 10%Bromophenol blue 0.0025%Deionized water (pH 8.6)Novex Tris-Glycine SDS Running Buffer Room temperature Tris base 25 mMGlycine 192 mMSDS 0.1%Deionized water (pH 8.3)Novex Tris-Glycine Native Running Buffer Room temperature Tris base 25 mMGlycine 192 mMDeionized water (pH 8.3)Novex Tris-Glycine Transfer Buffer Room temperature Tris base 12 mMGlycine 96 mMDeionized water (pH 8.3)* Tris-HCl solutions are prepared from Tris base and pH-adjusted with 6 N HCl.44'