b'Silver stainsUltrasensitive stains with optimized protocols and manufactured for minimal variabilitySilver staining is the most sensitive colorimetric method for detecting total protein, and functions by the deposition of metallic silver at the location of protein bands. Silver ions (from silver nitrate in the stain reagent) interact and bind with certain protein functional groups. The strongest interactions occur with carboxylic acid groups (Asp and Glu), imidazole (His), sulfhydryl groups (Cys), and amines (Lys). Various sensitizer and enhancer reagents are essential for controlling the specificity and efficiency of silver ion binding to proteins and effective conversion (development) of the bound silver to metallic silver.Key features: Sensitivesilver stains are highly sensitive stains that allow for visualization of proteins at subnanogram levels Easy to use and flexiblesilver stains are optimized forWe offer highly sensitive silver stains with short protocol minimal steps and have the flexibility to accommodatetimes that are also compatible with mass spectrometry. shorter or longer protocols The Invitrogen SilverXpress Silver Staining Kit providesWorkflow compatibleour mild chemical formulationsnanogram-level sensitivity with minimal background help ensure compatibility with mass spectrometry(Figures 38, 39), while the Thermo Scientific Pierce Silver and sequencing Stain Kit provides protocol flexibility (Figures 40, 41). Robust performancedetailed protocol enables consistent results with clear backgroundLearn more at thermofisher.com/silverstains67'