b'Protein extraction and clean-up methods Gentle formulations designed to maximize protein yield and activityObtain high protein yields from tissues, cells, or subcellular fractions using reagents and kits that are optimized for mammalian, bacterial, yeast, insect (baculovirus), and plant samples. These gentle formulations have been verified in multiple tissue types and cell lines, and generally eliminate the need for mechanical cell disruption.Overview of sample types and recommended protein extraction reagents and kits.Sample type Goal Recommended Thermo Scientific reagents or kitsM-PER Mammalian Protein Extraction Reagent T-PER Tissue Protein Extraction Reagent Primary cultured or mammalian cellsTotal protein extraction N-PER Neuronal Protein Extraction Reagent or tissuesRIPA Lysis and Extraction BufferPierce IP Lysis BufferNE-PER Nuclear and Cytoplasmic Extraction Reagents Subcellular fractionation kitsSubcellular fractionationMitochondria isolation kitsCultured mammalian cells or tissues or organelle isolation PierceCell Surface Protein Isolation KitSyn-PER Synaptic Protein Extraction ReagentLysosome Enrichment Kit for Tissues and Cultured CellsBacterial cells Total protein extraction B-PER Complete Bacterial Protein Extraction ReagentYeast cells Total protein extraction Y-PER Yeast Protein Extraction Reagent Y-PER Plus Dialyzable Yeast Protein Extraction Reagent Insect cells (baculovirus) Total protein extraction I-PER Insect Cell Protein Extraction ReagentPlant tissue (leaf, stem, root, flower) Total protein extraction Pierce Plant Total Protein Extraction KitDid you knowAlthough RIPA buffer is a common lysis buffer used for preparing samples for SDS-PAGE and western blot analysis, RIPA buffer is a strong lysis buffer than contains components that may interfere with certain protein assays and gel electrophoresis chemistries. Cross reference your RIPA buffer recipe to the interfering substances lists for both gel electrophoresis and protein assays, as multiple variants on the traditional RIPA buffer recipe exist.Learn more at thermofisher.com/proteinextraction37'