b'E-PAGE High-Throughput Precast Gel SystemProtein separation and analysis for increased sample throughputThe Invitrogen E-PAGE High-Throughput Precast Gel System is designed for fast, bufferless medium- and high-throughput protein analysis. Invitrogen E-PAGE 48-well and 96-well precast gels consist of a buffered gel matrix and electrodes packaged inside a disposable, UV-transparent cassette. Each cassette is labeled with a unique barcode to facilitate identification of the gel usingSpecificationscommercial barcode readers. These gels can be loaded Shelf life: 6 monthswith a multichannel pipettor or automated loading system. The E-PAGE system also includes Invitrogen E-Base Average run time: 23 minutesintegrated devices to run the gels, an E-Holder platform Separation range: 10200 kDa (Figure 24)for optional robotic loading, and free E-Editor 2.0 Software to align images for easy comparison.Polyacrylamide concentrations:Advantages of using the E-PAGE High-Throughput E-PAGE 48 gel: fixed 8%Precast Gel System include:E-PAGE 96 gel: fixed 6%Ease of usequick setup and fast protein separation in about 23 minutesGel dimensions: 13.5 x 10.8 cm (3.7 mm thick) Fast loadingcompatible with multichannel pipettors Maximum sample volume per well: and robotic loadingE-PAGE 48 gel: 20 L Efficient western blotting and stainingoptimized E-PAGE 96 gel: 15 L protocols and reagents A BGood to knowHow do E-PAGE gels work? CE-PAGE gels run in the Invitrogen E-BaseMother E-Base Device E-PAGE 96 gelselectrophoresis device, which has an integrated power supply for direct connection to an electrical outlet. Use the Invitrogen Mother E-Base DeviceDaughter E-Base Device for a single E-PAGE gel, or use the Mother E-Base Device in conjunction with two or more Invitrogen Figure 23. Loading and running E-PAGE gels. (A) Loading an Daughter E-Base Devices for running multipleE-PAGE 48 gel using a multichannel pipettor. (B) Loading an E-PAGE gels simultaneously (Figure 23). 96 gel using a multichannel pipettor. (C) The Mother/Daughter E-Base Device combination.Learn more at thermofisher.com/epage32'