b'XCell4 SureLock Midi-Cell (continued)A B1.Insert the XCell4 SureLock Midi-Cell assembly inNote: If you are having difficulty inserting the its unlocked position into the center of the midi-cellassembly into the lower buffer chamber, make sure base. The XCell4 SureLock assembly slides downthe cathode (black polarity indicator) of the buffer over the protrusion in the midi-cell base. core is aligned with the cathode (black polarity 2. Place one cassette on each side of the buffer coreindicator) of the lower buffer chamber.for each of the two cores. For each cassette, the4.The upper buffer chamber (cathode) is the void shorter well side of the cassette must face outformed between a gel and the buffer core at the towards the lower buffer chamber. center of each core.3. While holding the assembly together with your5.Lock the XCell4 SureLock assembly by moving the hands (A), insert the buffer cores with the geltension lever to the locked position (indicated on cassettes into the lower buffer chamber such thatthe XCell4 SureLock Assembly). This will squeeze the negative electrode fits into the opening in thethe gels and buffer cores together, creating gold plate on the lower buffer chamber (B). Alwaysleak-free seals.hold the cassette assembly by its edges as shown6. Proceed to loading samples and buffers.in the figure.Figure 30. How to use the XCell4 SureLock Midi-Cell with 4 gels. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20Figure 31. Quality of a precast Invitrogen NuPAGE 412% Bis-Tris Midi Gel with a variety of protein standards, lysates, and purified proteins. Electrophoresis was performed using MES running buffer and an XCell4 SureLock Midi Cell at 200V (constant). Following electrophoresis, the gel was stained using SimplyBlue SafeStain, destained using water, and imaged using a flatbed scanner. Sharp, straight bands were observed. Lanes 1, 10, 11, 20: 5L of Mark12 Unstained Standard (blend of 12 purified proteins); lanes 2, 9, 12, 19: 10g of E. coli lysate; lanes 3, 18: 6g of human IgG; lanes 4, 17: 6g of human IgM; lanes 5, 16: 5L of SeeBlue Plus2 Prestained Protein Standard; lanes 6, 15: 5L of Invitrogen BenchMark Protein Ladder; lanes 7, 14: 15L of Invitrogen MagicMark XP Western Protein Standard; lanes 8, 13: 5L of Invitrogen HiMark Unstained Protein Standard.60'