b'Silver stain kits.Pierce Silver StainSilverXpress Silver for Mass Spectrometry Pierce Silver Stain Kit Staining KitComponents (steps) 6 (7) 4 (7) 5 (9)Time required 1 hr 13 min 1 hr 30 min 2 hrLimit of detection 0.25 ng 0.25 ng 0.86 ngMass spectrometryYes Yes YescompatibleStorage Room temperature Room temperature 4CStability 1 year 1 year 6 monthsAdvantagesFast and sensitive staining and destaining Rapid, ultrasensitive, and Nanogram-level sensitivity for of protein gels versatile silver stain systemsilver staining with minimalOptimized for peptide recovery after in-gel Flexible gel fixation (30 minbackground trypsin digestion for mass spectrometry overnight) and staining (5 minFlexible gel fixation (1530 min to20 hr)overnight) and staining (130 min)Protocols and example dataH 2 O F SZ123456789 101. Wash gel with water. 2. Fix gel in Fixing Solution3. Decant Fixing Solution and for 10 minutes. incubate gel in 2 changes of Sensitizing Solution. H 2 O SS4. Decant Sensitizing Solution and rinse5. Incubate gel in Staining Solution. gel 2 times with ultrapure water. Figure 39. Crystal clear background with the SilverXpress Silver H 2 O D Staining Kit. Samples were separated on a NuPAGE 412% Bis-Tris gel and stained using the SilverXpress kit. Lanes 1, 10: Mark12 Unstained D Standard (blend of 12 purified proteins) diluted 1:4; lane 2: Mark12 Unstained Standard diluted 1:8; lane 3: Mark12 Unstained Standard 6. Decant Staining Solution and rinse7. Incubate gel in Developing Solution.diluted 1:16; lane 4: Mark12 Unstained Standard diluted 1:32; gel 2 times with ultrapure water.lane 5: Mark12 Unstained Standard diluted 1:64; lane 6: 1.6 ng BSA; lane 7: 0.8 ng BSA; lane 8: E. coli lysate diluted 1:20; lane 9: E. coli lysate SS diluted 1:80.H 2 O8. Add Stopping Solution directly to gel9. Decant Stopping Solution and wash when desired staining intensity is reached.gel 3 times with ultrapure water. Figure 38. SilverXpress Silver Staining Kit protocol. 68'