b'Novex Tricine buffer recipesBufferStorageComponentConcentration (1X) Novex Tricine SDS Sample Buffer +4C Tris-HCl* 450 mMGlycerol 12%SDS 4%Coomassie Blue G 0.0075%Phenol red 0.0025%Deionized water (pH 8.45)Novex Tricine SDS Running Buffer Room temperature Tris base 100 mMTricine 100 mMSDS 0.1%Deionized water (pH 8.3)* Tris-HCl solutions are prepared from Tris base and pH-adjusted with 6 N HCl.Zymogram buffer recipesBufferStorageComponentConcentration (1X) Novex Zymogram Plus Renaturing Buffer Room temperature Triton X-100 solution 2.7% (w/v) in H 2 ODeionized waterNovex Zymogram Plus Developing Buffer Room temperature Tris-HCI* 50 mMNaCl 200 mMCaCl 2 2H 2 O 5 mMBrij 35 0.006% (w/v)Deionized water _(pH 7.6)* Tris-HCl solutions are prepared from Tris base and pH-adjusted with 6 N HCl.Isoelectric focusing buffer recipesBufferStorageComponentConcentration (1X) Novex IEF Sample Buffer pH 37 4C Lysine (free base) 40 mMGlycerol15%Deionized water Novex IEF Sample Buffer pH 310 4C Arginine (free base) 20 mMLysine (free base) 20 mMGlycerol 15%Deionized water Novex IEF Cathode Buffer pH 37 4C Lysine (free base) 40 mM(upper buffer chamber) Deionized water Novex IEF Cathode Buffer pH 310 4C Arginine (free base) 20 mM(upper buffer chamber) Lysine (free base) 20 mMDeionized water (pH 10.1)Novex IEF Anode Buffer (for both pH ranges) Room Phosphoric acid 85% 7 mM(lower buffer chamber) temperature Deionized water (pH 2.4)Urea-thiourea-CHAPS 20C Deionized urea 7 M(rehydration buffer for IPG strips) Deionized thiourea 2 MCHAPS 24%Ampholytes 0.22.0%Bromophenol blue 0.002%Ultrapure water DTT 20 mM45'