b'Electrophoresis buffersWe offer reliable premixed SDS-PAGE buffers and reagents, including sample buffers, running buffers, reducing agents, and antioxidants.Buffer and reagent selection guideGel type Sample buffers optimized for use with the gel Running buffers optimized for use with the gelBolt Bis-Tris Plus gelBolt Sample Reducing Agent (10X) 20X Bolt MES SDS Running Buffer 4X Bolt LDS Sample Buffer (nonreducing)(3.5160 kDa separations) Bolt Antioxidant20X Bolt MOPS SDS Running Buffer(15260 kDa separations)NuPAGE Bis-Tris gelNuPAGE Sample Reducing Agent (10X)NuPAGE MES SDS Running Buffer (20X)4X NuPAGE LDS Sample Buffer (nonreducing) (3.5160 kDa separations) NuPAGE MOPS SDS Running Buffer (20X) NuPAGE Antioxidant(15260 kDa separations)NuPAGE Tris-Acetate gel4X NuPAGE LDS Sample Buffer (nonreducing)NuPAGE Tris-Acetate SDS Running Buffer (20X) NuPAGE Sample Reducing Agent (10X)Novex Tris-Glycine Native Running Buffer (10X)* Novex Tris-Glycine Native Sample Buffer (2X)*Novex Tris-Glycine gelNovex Tris-Glycine SDS Sample Buffer (2X)Novex Tris-Glycine SDS Running Buffer (10X) NuPAGE Sample Reducing Agent (10X)Novex Tris-Glycine Native Running Buffer (10X)* Novex Tris-Glycine Native Sample Buffer (2X)* Pierce Tris-Glycine SDS Buffer (10X) BupH Tris-Glycine Buffer PacksNovex Tricine gelNovex Tricine SDS Sample Buffer (2X)Novex Tricine SDS Running Buffer (10X)NativePAGE gelNativePAGE Sample Buffer (4X)NativePAGE Running Buffer (20X)NativePAGE 5% G-250 Sample Additive NativePAGE Cathode Buffer Additive (20X) Novex IEF gelNovex IEF Sample Buffer, pH 310 (2X) Novex IEF Anode Buffer (50X)IEF Sample Buffer, pH 37 (2X)Novex IEF Cathode Buffer, pH 310 (10X)Novex IEF Cathode Buffer, pH 37 (10X)Novex Zymogram Plus gel**Novex Tris-Glycine SDS Sample Buffer (2X)Novex Tris-Glycine SDS Running Buffer (10X)* Use this buffer when performing a native separation with this gel.** Novex Zymogram Developing Buffer (10X) and Novex Zymogram Renaturing Buffer (10X) are available for visualizing the Zymogram Plus gels.MES vs. MOPS running buffer Reducing agent Use MES SDS running buffersWhen preparing samples for reducing gel electrophoresis, any of the following to resolve small molecularreducing agents may be used:weight proteins. Bolt Sample Reducing Agent Use MOPS running buffers to resolve medium-sized proteins.NuPAGE Sample Reducing Agent MES has a lower pK athan MOPS, Dithiothreitol (DTT), 50 mM final concentration enabling gels with MES running -mercaptoethanol (-ME), 2.5% final concentrationbuffer to run faster than gels with MOPS SDS running buffer. Tris-(2-carboxyethyl)phosphine (TCEP), 50 mM final concentration The difference in ion migration affects stacking and results in aAdd the reducing agent to the sample up to an hour before loading the difference in protein separationgel. Avoid storing reduced samples for long periods, even if they are range between these buffers. frozen. Reoxidation of samples can occur during storage and produce inconsistent results.Learn more at thermofisher.com/electrophoresisbuffers42'