b'Novex Tricine gelsHigh-resolution gels for peptide analysis and low molecular weight proteinsThe Invitrogen Novex tricine gel system is a modification of the Tris-glycine system in which tricine replaces glycine in the running buffer. This system uses a discontinuous buffer system specifically designed for the resolution of low molecular weight proteins (Figure 16). Advantages of Novex Tricine gels over Tris-glycine gels include:Increased resolution of proteins with molecular weights as low as 2 kDa (Figure 17) Improved compatibility with direct protein sequencingapplications after transferring to PVDF membranes Minimized protein modifications, due to the lower pH ofthe tricine buffering systemGood to knowHow Novex Tricine gels workIn the traditional Tris-glycine protein gel system, the ults acquired wit resolution of smaller proteins (10 kDa) is hindered by Res hthe continuous accumulation of free dodecyl sulfate (DS) ions from the SDS sample and running buffers in the stacking gel, which causes mixing of the DS ions the Mini Gel Tank with smaller proteins and results in fuzzy bands and Figure 16. Novex Tricine gel electrophoresis. Protein standardsdecreased resolution. The mixing also interferes with and samples were loaded at 10 L sample volumes on Invitrogenthe fixing and staining of smaller proteins. The Novex Novex 1020% Tricine Protein Gels. Electrophoresis was performedTricine gel system uses a low pH in the gel buffer and using the Mini Gel Tank at 200 V (constant). Sharp, straight bands were observed after staining with SimplyBlue SafeStain. Image was acquiredsubstitutes tricine for glycine in the running buffer. using a flatbed scanner. Lane 1: SeeBlue Plus2 Prestained Standard;The smaller proteins and peptides that migrate with lane 2: 10 g E. coli lysate; lane 3: Mark12 Unstained Standard (blendthe stacked DS ions in the Tris-glycine gel system are of 12 purified proteins); lane 4: 40 g HeLa cell lysate; lane 5: 20 g HeLa cell lysate; lane 6: 5 g BSA; lane 7: 40 g Jurkat cell lysate;well separated from DS ions in the Novex Tricine gel lane 8: 5 g GST fusion protein; lane 9: Novex Sharp Unstained Proteinsystem, offering sharper bands and higher resolution.Standard; lane 10: 5 g -galactosidase.Review the protocol for Novex Tricine gels here. Learn more at thermofisher.com/tricine24'