b'Novex Tris-Glycine gelsLoad up to 60 L of sample The Invitrogen Novex Tris-Glycine mini gels, WedgeWell format, and Invitrogen Novex Tris-Glycine Plus midi gels are polyacrylamide gels based on traditional Laemmli chemistry that enable the use of Laemmli sample and running buffers. Novex Tris-Glycine gels provide high-quality performance and separation of a wide range of proteins into well-resolved bands (Figures 11 and 12).Highlights: Wedge-shaped wellseasily load up to 60 L of sample without sacrificing gel width or length (mini gel only) High performanceexcellent protein band resolution and sharpness Improved shelf lifestore gels for up to 12 months at 4C Fast run conditionsquickly separate your proteins using constant voltage in less than 60 minutes Flexiblecompatible with native and denatured protein samples1 2 3 4 5 6 7 8 9 10 1 2 3 4 5 6 7 8 9 10 esults acquired witR hthe Mini Gel TankNovex 420% Tris-Glycine Mini Gel, Bio-Rad TGX 420% GelWedgeWell formatFigure 11. Better protein resolution and band sharpness with Novex Tris-Glycine Mini Gels, WedgeWell format. Protein ladder, purified proteins, and E. coli lysate were loaded on a gradient Novex 420% Tris-Glycine Mini Gel, WedgeWell format, and a Bio-Rad TGX 420% gradient gel. The Bio-Rad TGX gel displays numerous low molecular weight protein degradation products below major bands in lanes 3, 4, 7, 8. These are not seen in the Novex Tris-Glycine gel. The Novex gel also displays better protein band sharpness and resolution of lysate than the Bio-Rad gel. Lanes 1, 10: 5 L Mark12 Unstained Standard; lane 2: 10 g E. coli lysate; lane 3: 6 g catalase; lane 4: 6 g carbonic anhydrase; lane 5: 6 g lysozyme; lane 6: 6 g hIgM; lane 7: 6 g BSA; lane 8: 6 g -galactosidase; lane 9: 20 g E. coli lysate. The sample volume for lanes 29 was 10 L.Review the quick reference protocol for the Novex Tris-Glycine mini gels, WedgeWell format, here.Learn more at thermofisher.com/novexwedge20'