b'Novex IEF gelsPrecast gels for isoelectric point determinationIsoelectric focusing (IEF) is an electrophoresis techniqueSpecificationsthat separates proteins based on their isoelectric point (pI). Shelf life: 2 monthsThe pI is the pH at which a protein has no net charge and does not move in an electric field. Invitrogen Novex IEF Average run time: 2.5 hoursgels effectively create a pH gradient so proteins separate Separation range:according to their unique pI (Figures 20 and 21). These gels can be used for pI determination or for detection of minor pH 310 gels: pI performance range is 3.58.0changes in a protein due to deamination, phosphorylation, pH 37 gels: pI performance range is 3.07.0or glycosylation, and can resolve different proteins of similar size that cannot be resolved on standard SDS-PAGE gels.Polyacrylamide concentration: fixed 5%When used with our convenient, preoptimized Gel dimensions: 8 x 8 cm (1 mm thick)buffers, solubilizers, and molecular weight markers, Maximum sample volume per 10-well gel: 20 LNovex IEF gels can provide: Accurate pI determination Clear, sharp bands for easy identification ofprotein modifications Higher resolution of slight differences in size when usedin combination with SDS-PAGE for 2D electrophoresispl 1 2 3 4 5 6 7 8 9 108.07.4esults acquired wit6.0 R h4.5the Mini Gel TankFigure 20. Novex IEF gel electrophoresis. A 2-fold dilution series of Invitrogen IEF Marker 310 was run in duplicate on an Invitrogen Novex pH 310 IEF Protein Gel using a Mini Gel Tank. The IEF Marker 310 consists of proteins with a variety of isoelectric points; these proteins include lectin (pI = 7.8, 8.0, and 8.3), myoglobin from horse muscle (pI = 6.9 and 7.4), carbonic anhydrase from bovine erythrocytes (pI = 6.0), -lactoglobulin from bovine milk (pI = 5.2 and 5.3), soybean trypsin inhibitor (pI = 4.5), and glucose oxidase (pI = 4.2). After electrophoresis, the gel was fixed and stained using Coomassie R-250 dye. Gel imaging was performed with a flatbed scanner. Volumes of IEF Marker 310 loaded: Lanes 1, 6: 20 L; lanes 2, 7: 10 L; lanes 3, 8: 5 L; lanes 4, 9: 2.5 L; lanes 5, 10: blank.Learn more at thermofisher.com/ief28'