b'Novex Tris-GlycineNovex Tris-Glycine gelsgel specifications Shelf life: up to 12 months at 4CNovex Tris-Glycine gels, WedgeWell formatLarge proteins Midsize proteins Small proteins Wide range Average run time: 60 minutes (116500 kDa) (20250 kDa) (360 kDa) (6200 kDa) Separation range: 8 kDa to 260 kDa 6%8% 10% 12% 14% 16%412% 816% 420%1020% Polyacrylamide concentrations: fixed 6%, 8%, 10%, 12%, 14%, 16%; 10 200 kDagradient 412%, 420%, 816%, 1020%200 kDa200 kDa 200 kDa 116 kDa Gel dimensions:20 97 kDa200 kDa 116 kDa97 kDa 200 kDa 200 kDa Mini: 8 x 8 cm (1 mm thick) 200 kDa 116 kDa 116 kDa97 kDa 66 kDa30 97 kDa 66 kDa Midi: 8 x 13 cm (1 mm thick) 200 kDa116 kDa 66 kDa 55 kDa 116 kDa55 kDa Maximum sample volume: 97 kDa 66 kDa 116 kDa 97 kDa40 200 kDa 55 kDa In 10-well mini gel, WedgeWell116 kDa 55 kDa 36 kDa 97 kDa 66 kDaPercent length of gelformat: 60 L 66 kDa50 97 kDa 116 kDa 36 kDa In 20-well midi gel with standard36 kDa 31 kDa 66 kDa 55 kDawell format: 25 L55 kDa 97 kDa 31 kDa55 kDa60 36 kDa 31 kDa66 kDa 21 kDa 66 kDa 36 kDa116 kDa70 31 kDa 31 kDa 21 kDa55 kDa 36 kDa97 kDa 21 kDa 14 kDa55 kDa 36 kDa 31 kDa21 kDa 14 kDa8014 kDa 36 kDa 21 kDa 6 kDa31 kDa 21 kDa 6 kDa 14 kDa90 31 kDa 14 kDa6 kDa 6 kDa66 kDa 6 kDa21 kDa36 kDa100Figure 12. Migration patterns of a protein standard on Novex Tris-Glycine gels, WedgeWell format. Use this chart to select the proper gel for separating proteins based on size. Optimal resolution is achieved when protein bands migrate within the shaded regions. The standard represented here is the Mark12 Unstained Standard under denaturing conditions. Recommended productsFor sample clean-up prior to electrophoresis, weBuffers for native proteins: Invitrogen Novex recommend using the Thermo Scientific PierceTris-Glycine Native Sample Buffer and Tris-Glycine SDS-PAGE Sample Prep Kit. Native Running Buffer.Buffers for denatured proteins: Invitrogen NovexPageRuler, PageRuler Plus, and Spectra protein Tris-Glycine SDS Sample Buffer and Tris-Glycine SDSladders are recommended for molecular weight Running Buffer. determination with Novex Tris-Glycine gels.21'