b'SureLock Tandem Midi Gel Tank troubleshootingObservation Possible cause Suggested solutionBuffers were too dilute Check buffer recipes. Do not reuse buffers. Remake buffer if necessary.Buffer chamber was leaking Make sure the cassette clamp is firmly seated, the gaskets are in place, Run takes longer than normal and the cassette clamps are locked.Voltage and/or current was setSet the correct voltage and/or current. See Running conditions in the too low Gel electrophoresis protocol (p. 10) of the user guide for more details.Incorrect running buffer was used orCheck buffer recipe. Dilute or remake buffer if necessary.Run is faster than normal,buffer was too concentratedwith poor resolution Voltage and/or current was setDecrease voltage and/or current to recommended running conditions. See too high Running conditions in the Gel electrophoresis protocol (p. 10) of the user guide for more details.Tape was left on the bottom ofRemove the tape from the cassette.the cassetteCheck conductance on all connections using a voltmeter.Connection to power supply was not complete Note: Do not check connections if not trained in this procedure. Current reading on powerContact Technical Support for further help.supply is zero or very low Make sure there is sufficient buffer in the tank. The cathode (inside) chamber needs to be filled to above the wells (~170 mL), and Insufficient buffer level the anode (outside) chamber needs to be filled to the red fill line (~350 mL). See Fill tank with running buffer and load samples in the Gel electrophoresis protocol (p. 12) of the user guide for more details.Cannot see the sample wellsDifficult to see contrast between theMark cassette at the bottom of the wells with a marker pen prior to placing to load the sample sample well and the rest of the gel the cassette in the electrophoresis tank.Too many gels were being run at onceCheck the power limits of the power supply being used and use additional using one power supply power supplies if needed.Ensure that the cassette clamp is firmly seated, the gaskets are in place, Cassette was not properly clamped and the cassette clamps are in the locked position. See Insert cassette Current reading on powerin the Gel electrophoresis protocol (p. 11) of the user guide for details.supply is much higher thanIf the buffer level is above the height of the gel, the cathode and anode expected, or maxed out chambers are in direct contact, resulting in an electrical short circuit. Ensure correct buffer volumes. The cathode buffer level should be covering Tank was overfilled with running buffer the wells but not above the height of the gel (~170 mL). The anode buffer level should be filled to the red fill line marked on the outside of the tank (~350 mL). See Fill tank with running buffer and load samples in the Gel electrophoresis protocol (p. 12) of the user guide for more details.The cassette is installed backwardsInstall the cassette in the correct orientation, with the well opening facing Proteins are not migratingin the tank toward the cathode (center of the tank).into the gel The power cable is installedInstall the power cable in the correct orientation (red to red, black to black).backwards into the power supply78'