b'Acetic Lysate staining MW marker stainingEtOH acidH 2 O FF1. Wash 2 x 5 minutes 2. Fix 2 x 15 minutes in EtOH/acetic acid.with ultrapure water.10% EtOH SZSZ3. Incubate 2 x 5 minutes 4. Mix Sensitizer. Sensitize for 1 minute.2 minutes, 30 seconds 2 minutes, 30 secondswith 10% EtOH. Wash. Wash 2 x 1 minute. Development timeFigure 41. The Pierce Silver Stain Kit exhibits excellent sensitivity. In standard mini gels, proteins are detectable at greater than 0.25 ng per S band or spot.S5. Mix Silver Stain. Stain for 5 minutes.Wash 2 x 20 seconds.D 5% acetic acidD6. Mix Developer. Develop for 23 minutes.7. Remove Developer. Stop with 5% acetic acid for 10 minutes.Figure 40. Pierce Silver Stain Kit protocol.69'