b'Coomassie dyebased protein gel stainsConvenient, ready-to-use reagents with no permanent chemical modificationThe most common methods of in-gel protein detection use stains with Coomassie dye. These stains use either the G-250 (colloidal) or R-250 form of the dye. Colloidal Coomassie stain can be formulated to effectively stain proteins within one hour and require only water (no methanol or acetic acid) for destaining. Key features: SimpleCoomassie dyebased formulations are easy to formulate and are widely used Easy to usesimply soak the gel in staining solution, and destain to observe protein bands EconomicalCoomassie dyebased formulations are cost-effective Our Coomassie stains provide sensitive protein detection along with simplified protocols. Staining protocols andFlexibleuseful for qualitative visualization, quantitativeexample data are shown for SimplyBlue SafeStain densitometry, and gel excision and analysis by (Figures 32, 35, 36), Imperial Protein Stain (Figures 33, 37), mass spectrometry and PageBlue Protein Staining Solution (Figure 34).Coomassie dyebased protein gel stains.SimplyBlue SafeStain Imperial Protein Stain PageBlue Protein Staining SolutionType G-250 R-250 G-250Limit of detection 7 ng 3 ng 5 ngTime to stain* 12 min 12 min 30 minCompatible with:PVDF membranes Yes Yes YesNitrocellulose membranes No No NoReusable No No Yes (up to 3 times)Mass spectrometryYes Yes YescompatibleColor Purple Purple Blue-greenFeature Free of methanol and acetic acid Photographs better thanFree of methanol and acetic acidCoomassie G-250 dyeAdvantages Rapid, sensitive, completelyFast, ultrasensitiveCost-effective option for fast, nonhazardous staining (does notprotein detection sensitive stainingrequire methanol or acetic acid fixatives or destains)* Approximate staining time using microwave ovenLearn more at thermofisher.com/coomassiestains64'